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A total of 452 street sputum specimens were collected in the areas of Daegu city and tested for the isolation of mycobacteria. Sputa were treated with 4% sodium hydroxide(NaOH) for 20 minutes and inoculated onto 3% acid-buffered OGAWA medium, and incubated at 37℃ for a period of eight weeks. Tests employed for the identification of the mycobacterial strains isolated were : 1) Growth rate and temperature, 2) Staining property, 3) Colonial morphology, 4) Pigmentation, 5) Growth on 500 ㎍ p-nitrobenzoic acid, 6) Niacin production 7) 5% NaCl tolerance, 8) Growth on 5 ㎍ ethambutal (EB), 9) Nitrate reduction, and 10) Catalase activity pH7.0/68℃ for 20 minutes. Considering that tuberculosis still remains a serious health problem in Korea, the isolation of M tuberculosis from the street sputum, though it showed the recovery rate 1.7% out of the total tested brings to the particular public attention on health education.
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